PacBio REVIO Long-read Sequencing

Home > Services > PacBio REVIO Long-read Sequencing

PacBio REVIO Long-read Sequencing

LONG-READ NEXT GENERATION SEQUENCING AT SCALE

REVIO long-read sequencing services help you achieve more with less DNA input. HiFi reads combine long-read length with high accuracy from parallel sequencing of four independent stages, and it is fully automated.

The PacBio REVIO system utilizes nanofabricated Revio SMRT^® Cells, each of which has 25 million zero-mode waveguide wells.

The REVIO HiFi sequencing system best utility is for genome assembly, DNA methylation, RNA methylation, structural variation detection, and long-read transcriptome analysis with uniform coverage.

Inquire about SeqMatic’s long-read sequencing solutions. An expert can help you optimize your workflow goals with system recommendations catered to your analysis needs.

Service Highlights
Key Applications & Sample Throughput with SPRQ chemistry

Long-read Sequencing Service Highlights

Expert Consultation – Our scientists can help you select the most scientifically viable and cost-effective parameter choices for your run design, sample load and barcode assignment requirements
Comprehensive Services – High Molecular Weight (HMW) extractions, FEMTO Pulse HMW gDNA visualization, shearing on a Megaruptor 3, and all SAGE size selection services. Includes library preps from amplicons, gDNA, total RNA, mRNA, and single cells using all available PacBio kits, Kinnex Arrays, customized Kinnex arrays, custom workflows and also offer low input library preps.
Stringent Quality Controls – All sample preparation and library construction steps are validated by multiple assays (Qubit, FemtoPulse)
Technical Support – QC reports prepared for review by the customer to decide which samples qualify for library preparation, or sample re-submission
Flexibility – Choice for sample processing: manually or automated using a range of processing technologies
Wide-Range of Sample Types – human, animal, fish, from virtually any source

Revio System Applications

The Revio system supports a variety of applications that benefit from accurate long HiFi reads and requires only 500 ng of input DNA per sample.

Library	Application	Per Revio SMRT Cell	Per Year^*	Expected Coverage^†
0.5–5 kb	Amplicon sequencing	>1,000	>2.5 million	50×
5–10 kb	Microbial genome	384	480,000	30×
5–15 kb	PureTarget panels	96	96,000	>100× mean per target
5–10 kb	Ampli-Fi human genome	1	1,2002	20×
15–20 kb	Human genome	2	2,500	20×
15–20 kb	Human methylation profiling	8	10,000	5×
15–20 kb	Transcriptome with Kinnex™ full-length RNA kit	6	7,500	10 million reads

*Annual throughput is based on 2,500 Revio SMRT Cells for 12 hour runs; 1,250 for 24 hour runs; and 1,050 for 30 hour runs. Adaptive loading — which increases the consistency of yield per SMRT Cell — adds about 4 hours to run time, affecting the maximum number of SMRT Cells per year.

† Expected coverages are estimates.

Ultima Genomics UG 100 Sequencer

Oxford Nanopore MinION Long-read Sequencing

Service Portfolio

PRODUCT HIGHLIGHT

TailorMix Dual-Indexed PhiX

Check Product Highlights