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Methylation

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Methylation (Methyl-Seq) Analysis Services

Enzymatic Methylation – insights at a single nucleotide level

Methylome sequencing analysis services offer crucial understandings of the intricate processes behind gene control, and how these impact wellness, illness, and the ability to adapt to environmental changes.

SeqMatic offers NEBNext® Enzymatic Methyl-seq (EM-seq) services for identification of 5-methylcytosine (5nC) and 5-hydroxymethylcytosine (5hmC) within genomes. This high-performance methylome analyses method provides high-quality libraries with less fragmentation, less biases and minimal loss or damage to DNA when compared to bisulfite methylome analysis using Illumina sequencing.

Methyl-seq has several advantages, including the ability to capture full sample diversity with small amounts of DNA and covers emerging regions of interest in the human genome. At SeqMatic, Methyl-seq applications include studies of disease states (i.e. cancer); monitoring of embryonic development; studies of agricultural plants, etc.

Enzymatic Methlylation Advantages:

Superior sensitivity of detection of 5-mC and 5-hmC
Greater mapping efficiency
More uniform GC coverage
Detection of more CpGs with fewer sequence reads
Uniform dinucleotide distribution
High-efficiency library preparation, with larger library insert sizes

OSample Requirements:

Examples of SeqMatic experience with non-model organisms include:

200 ng input genomic DNA suggested for best results
A minimum of 10ng input is required per sample

We can process full workflows or accept your tissue, fresh/frozen cells/nuclei, or prepared libraries.

Your S.F. Bay Area Advantage!

To maintain maximum sample viability, your samples can be transported to us
within 1 to 2 hours from any where in the SF Bay Area – Request a Quote!

SeqMatic’s Premium ServicesOnsite-Seq^TM brings our experts to your lab to generate the GEMS. Fetch-Seq^TM courier pick-up in the S.F. Bay Area

Schupp, Patrick Georg. Clarifying the Transcriptional Profiles of Malignant Clones and Nonmalignant Cells of the Microenvironment through Multiscale and Multiomic Analysis of Individual Tumors. Diss. UCSF, 2023.

Barker, Scarlett J., et al. “Targeting Transferrin Receptor to Transport Antisense Oligonucleotides Across the Blood-Brain Barrier.” bioRxiv (2023): 2023-04.

Nair, Anup K., et al. “Generation of Isogenic hiPSCs with Targeted Edits at Multiple Intronic SNPs to Study the Effects of the Type 2 Diabetes Associated KCNQ1 Locus in American Indians.” Cells 11.9 (2022): 1446.

Find other Research Publications from around the world!

Your S.F. Bay Area Advantage!

To maintain maximum sample viability, your samples can be transported to us
within 1 to 2 hours from any where in the SF Bay Area – Request a Quote!

Barker, Scarlett J., et al. “Targeting Transferrin Receptor to Transport Antisense Oligonucleotides Across the Blood-Brain Barrier.” bioRxiv (2023): 2023-04.

Find other Research Publications from around the world!

DISCLAIMER: Workflow images and descriptive content on this page are from New England BioLabs Inc. Application Note: NEBNext^® Enzematic Methyl-seq (EM-seq^TM).

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