RNA Sequencing

Exosome

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Exosome RNA sequencing analysis plays a crucial role in unveiling the molecular makeup of exosomes, which are tiny extracellular vesicles carrying a diverse range of cargo, including miRNA, mRNA, lncRNA, lipids, and proteins. These versatile exosomes serve specific functions in various physiological processes, such as blood coagulation, waste management, and cellular communication. The non-coding RNAs within exosomes are believed to be key players in regulating molecular activities in recipient cells.

Exosome RNA Sequencing Services

Detection of Genetic Mutations

SeqMatic utilizes state-of-the-art RNA sequencing technologies to accurately characterize the comprehensive RNA content of exosomes, covering both protein-coding and lncRNA transcripts. Hence, our exosome RNA sequencing services are a great option to consistently demonstrate the existence of intricate RNA cargoes within exosome populations released by different cell types. For example, exosomes along with other extracellular vesicles, have been found in biofluids such as saliva, blood, urine, and cerebrospinal fluid.

Service Features

Stringent Quality Controls –

SeqMatic qualifies RNA with an Agilent TapeStation Electropherogram for each RNA sample submitted. A TapeStation analysis includes a RNA Integrity Number (RIN) and DV200 for each sample, which establishes relative length as a measure of RNA quality. Results are shared for review and approval prior to proceeding to library preparation. Gel cut purification and size selection are available.

High-Quality design considerations for read depth –

SeqMatic suggests typical read depth of 30M reads per sample. Higher and lower read depths are available depending on project needs

Preliminary Sequencing –

After library preparation, libraries are pooled in equal volumes and the final pool is quantified by Agilent TapeStation and checked via preliminary sequencing (MiSeq). Libraries are then normalized and re-pooled based on the read number from the preliminary sequencing dataset.

World-class validation standard & practices –

SeqMatic is equipped to ensure accurate, repeatable and reproducible results. SeqMatic’s quality program has been audited and approved as part of becoming a Clinical Laboratory Improvement Amendments (CLIA) and College of American Pathologists (CAP) certified sequencing facility. Our CLIA quality program requires the use of well-defined protocols to maintain the consistency and reproducibility of our NGS workflows. Thus, the samples will be processed in a CLIA environment.

Custom assay services –

Our highly personalized sequencing services can be adapted to almost any (high and/or low throughput) sequencing workflow required by our customers so that we optimize the quality and quantity of data output.

Service Highlights