PCR Amplicons & Plasmid Sequencing Services

PCR Amplicon Sequencing

• Library Preparation services available
• Long amplicons can be sequenced at full length using MiSeq 500 and 600 cycle kits and the NovaSeq SP500
• NovaSeq 6000 and NovaSeq X are available for high-throughput projects (up to 384 unique samples)
• TailorMixTM Indexed PhiX controls to address low diversity
• Consultation for staggered sequencing to maximize diversity by request
• Your validated custom primers can be used for two-step PCR or during sequencing

Fast Turnaround Time

• 3 to 5 business days Express Service for prepared libraries
• 10 business days Express Service for library preparation
• Pooling and Normalization of pre-made libraries are available

Stringent Quality Controls

SeqMatic qualifies DNA with a TapeStation Electropherogram and Qubit for each total DNA sample submitted. A TapeStation analysis includes a DNA Integrity Number (DIN) for each sample, where a value of 10 indicates a perfectly intact sample and a value of 1 indicates a severely degraded sample. Results are shared for review and approval prior to proceeding to library preparation.

High-Quality Design Considerations for Read Depth

SeqMatic offers typical read depth of 50K to 100K or more reads per sample. Higher and lower read depths are available depending on project needs.

Preliminary Sequencing

After library preparation, libraries are pooled in equal volumes and the final pool is quantified by Agilent TapeStation and checked via preliminary sequencing (MiSeq). Libraries are then normalized and re-pooled based on the read counts from the preliminary sequencing dataset.

World-Class Validation Standards & Practices

SeqMatic is equipped to ensure accurate, repeatable, and reproducible results. SeqMatic’s quality program has been audited and approved as part of becoming a Clinical Laboratory Improvement Amendments (CLIA) and College of American Pathologists (CAP) certified sequencing facility.

Our CLIA quality program requires the use of well-defined protocols to maintain the consistency and reproducibility of our NGS workflows. Thus, samples are processed in a CLIA environment.

Custom Assay Services

Our highly personalized sequencing services can be adapted to almost any high- and/or low-throughput sequencing workflow required by our customers so that we optimize the quality and quantity of data output.