Spatial Transcriptomics Services

The Visium Spatial Gene Expression platform employs PolyA capture technology, making it versatile across species and free from bias. SeqMatic also offers the Visium CytAssist platform, which uses specialized probes to sequence both fresh frozen tissue and FFPE samples from human and mouse specimens.

Diagram description: In a Visium direct placement workflow, a fresh frozen tissue section is placed onto a Visium gene expression slide and imaged for histological purposes (either H&E for morphological context, or IF for protein co-detection). Each Capture Area on a Visium slide has an array that captures mRNA. The spatial barcodes are added to the mRNA. The libraries are then sequenced and data visualized to determine which genes are expressed, and where, as well as in what quantity.

The spatial transcriptomics platform Visium CytAssist has a pre-set of probes (human or mouse) which allows the use of fresh frozen or FFPE tissue sections. The advantage of automation is that it uniformly distributes and transfers the probe set and provides consistency within the workflow. Custom probe sets can also be designed for use with the assay.

Learn more about SeqMatic’s CytAssist Services.

Diagram description: The Visium CytAssist workflow begins on a standard glass slide, where researchers perform sectioning, tissue preparation, and either H&E or IF staining, followed by imaging. Next, after probe hybridization, two standard glass slides and a Visium slide with two Capture Areas are placed in the CytAssist instrument so that the tissue sections on the standard slides can be aligned on top of the Capture Areas. Within the instrument, a brightfield image is captured to provide spatial orientation for data analysis, followed by hybridization of transcriptomic probes to the Visium slide. The remaining steps, starting with probe extension, follow the standard Visium workflow outside of the instrument.

SeqMatic expertly processes frozen and formalin-fixed paraffin-embedded (FFPE) tissues while also providing custom processing solutions for single stain chromogenic and immunofluorescent services. Through these services, the company helps customers optimize and validate antibodies, then generates detailed scans and comprehensive reports.